Run flux balance analysis (FBA/FVA) on genome-scale metabolic models (E. coli core, Recon3D, AGORA2) with COBRApy; simulate single/double gene knockouts and integrate RNA-seq expression via GIMME/iMAT. Use when predicting metabolic fluxes, finding essential genes or drug targets, doing synthetic let
Run flux balance analysis (FBA/FVA) on genome-scale metabolic models (E. coli core, Recon3D, AGORA2) with COBRApy; simulate single/double gene knockouts and integrate RNA-seq expression via GIMME/iMAT. Use when predicting metabolic fluxes, finding essential genes or drug targets, doing synthetic lethality screens, or building transcriptomics-constrained metabolic models from SBML/JSON GEMs.