Parse/write FASTA, FASTQ, SAM/BAM, VCF, BED, GFF/GTF with pysam and pure Python; decode SAM FLAG/CIGAR; reconcile 0-based vs 1-based coordinates. Use for custom format parsers or off-by-one coordinate bugs.
Parse/write FASTA, FASTQ, SAM/BAM, VCF, BED, GFF/GTF with pysam and pure Python; decode SAM FLAG/CIGAR; reconcile 0-based vs 1-based coordinates. Use for custom format parsers or off-by-one coordinate bugs.
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