bio-applied-ont-processing

SKILLWorkflowcommunity
v0.0.0Pavel-KravchenkoUnknownUpdated 1mo agoSource →

Basecall ONT POD5/FAST5 signal with Dorado (fast/hac/sup, duplex, 5mC/5hmC), QC with NanoStat/NanoPlot, filter with NanoFilt, and align with Minimap2 map-ont. Use for nanopore raw-signal processing, Q-score/length read filtering, N50 computation, or a POD5-to-aligned-BAM pipeline.

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1mo agoLast update
Skill
AuthorPavel-Kravchenko
Version0.0.0
LicenseUnknown
CategoryWorkflow
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About

Basecall ONT POD5/FAST5 signal with Dorado (fast/hac/sup, duplex, 5mC/5hmC), QC with NanoStat/NanoPlot, filter with NanoFilt, and align with Minimap2 map-ont. Use for nanopore raw-signal processing, Q-score/length read filtering, N50 computation, or a POD5-to-aligned-BAM pipeline.

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